FastQCFastQC Report
Sun 11 Jun 2023
HPAP-018_beta_98579_FGC2155_s_1_CGCTCATT-ATAGAGGC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-018_beta_98579_FGC2155_s_1_CGCTCATT-ATAGAGGC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24401683
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG819510.3358415892870996No Hit
CTCTCGGTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCTA799020.3274446274873746No Hit
CTCTCGGTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCTA775010.31760514223547615No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT623370.25546188760832605No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT524330.21487452320399378No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA501550.2055391015447582No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT430300.17634029587221503No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG392000.16064465717385149No Hit
TGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCC384630.15762437369586352No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT346950.14218281583282597No Hit
GCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGT325130.13324080966054677No Hit
CTCTTGGTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCTA322540.1321794074613624No Hit
CTCTTGGTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCTA320780.13145814573527573No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG282970.11596331285837949No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG280340.1148855183472386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)